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目的 :观察阿霉素肾病组织环孢素亲合素基因的表达。 方法 :应用传统的方法首先建立阿霉素肾病模型 ,通过逆转录聚合酶链反应 ( RT- PCR)测定阿霉素肾病肾组织环孢素亲合素基因的表达。 结果 :8只 SD大鼠经阿霉素注射后 ,尿蛋白 1周后开始上升 ,2周后明显增加 ,4周后尿蛋白均 >10 0 mg/2 4h;对照组尿蛋白始终在 6mg/2 4h以下。阿霉素肾病肾组织环孢素亲合素基因表达比对照组明显增高。 结论 :说明阿霉素肾病肾组织高水平的环孢素亲合素基因表达是肾组织损伤的原因之一 ,对 Cy P在自身免疫性疾病和器官移植排斥中的研究具有重要的理论与应用价值
Objective: To observe the expression of cyclosporin avidin gene in adriamycin nephropathy. Methods: The adriamycin nephropathy model was established by traditional method. The expression of cyclosporin gene in adriamycin-induced nephropathy was assayed by reverse transcription-polymerase chain reaction (RT-PCR). Results: After 8 weeks of adriamycin injection, urinary protein began to increase in 8 SD rats and increased significantly after 2 weeks. Urine protein was> 100 mg / 2 4 h after 4 weeks. Urinary protein in the control group was always at 6 mg / 2 4h or less. Adriamycin nephropathy kidney cyclosporin gene expression was significantly higher than the control group. Conclusion: The high level of cyclosporin gene expression in adriamycin-induced nephropathy kidney is one of the causes of renal tissue injury. There is an important theory and application of Cy P in the study of autoimmune diseases and organ transplant rejection value