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目的 获取较高纯度的神经前体细胞 (NPC)。方法 加入 30ng/mlbFGF培养E12天大鼠皮质、海马和隔区的NPC ,用免疫组化与免疫荧光组化方法将其显示。结果 加bFGF组的NPC不断分裂 ,形成大量的分裂球并维持到第 4代 ;绝大部分细胞 (约 98%以上 )表达Nestin ,约有 6 0 %为BrdU和Nestin免疫荧光双标阳性细胞。对照组的分裂球维持时间较短 ,随培养时间延长细胞向外迁移生长 ;第 2代约有 6 0 %细胞表达Nestin ,40 %为BrdU和Nestin免疫荧光双标阳性细胞。结论 bFGF对E12天大鼠脑组织的NPC有明显的促分裂作用 ,选用胎龄较小的胚脑组织加入适量的bFGF培养可获取较高纯度的NPC。
Objective To obtain higher purity of neural precursor cells (NPC). METHODS: NPCs in the cortex, hippocampus and septal area of E12 rats were cultured with 30ng / ml bFGF, and were expressed by immunohistochemistry and immunofluorescence staining. Results The NPCs in the bFGF group were divided into two groups. The majority of cells (more than 98%) expressed Nestin and about 60% were positive double-labeled BrdU and Nestin immunofluorescence. In the control group, the spheroplasts maintained for a short time and migrated outwardly with the incubation time. About 60% of the cells in the second generation expressed Nestin and 40% were BrdU and Nestin immunofluorescence double-labeled positive cells. Conclusion bFGF can promote the NPC of E12-day-old rat brain. Using the embryonic brain tissue with the smaller gestational age, bFGF can obtain higher purity of NPC.