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云南民族草药臭灵丹广泛用于治疗上呼吸道感染,疗效得到临床初步验证,但相关基础实验报道甚少.臭灵丹气味浓烈,民族用药经验是鲜品泡服,倾向保留挥发油成分,提示挥发油很可能有药用价值.目的:验证臭灵丹挥发油的体内外抗流感药效.方法:(1)以MDCK细胞、细胞病变抑制法在治疗模式下研究臭灵丹挥发油对H1N1流感病的抑制作用;(2)以流感小鼠研究臭灵丹挥发油对流感病毒感染肺炎的抑制作用.结果:(1)体外抗流感实验显示臭灵丹挥发油TC50 (mg/ml) ＜0.01％(浓度),IC50 (mg/ml》 ＞0.01％(浓度),SI＜1,提示无效;(2)流感小鼠肺炎抑制实验显示,臭灵丹挥发油低剂量组(体积比:2％)、高剂量组(体积比:8％)的肺指数分别为1.74±0.08、1.58±0.13,与病毒组比较(肺指数:2.12±0.28)有统计学差异(P＜0.05).结论:灵丹挥发油体内对流感有抑制作用,但体外无药效,提示其抗流感机制并非直接抑制病毒蛋白.“,”Laggera pterodonta (DC.) Benth has been used as folk medicine to treat upper respiratory tract infection in Yunnan province.Its curative effect has been preliminarily verified by clinical trials,but few of experimental research reports have been related.Laggera pterodonta (DC.) Benth,having powerful taste,is infused fresh in ethnical clinical experience,which has been proven by our survey in Yunnan.That provided us enlightenment that the volatile oil in it is likely to have medicinal value.Our experiments have shown that its volatile oil can suppress inflammation in mice with pneumonia induced by influenza,but has no inhibitory effect in flu cells.It suggests that its effect of treatment of influenza maybe not because of direct antiviral.