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经三步低速离心分层提纯，使恶性体腔积液的肿瘤细胞得到了尽可能地浓集，同时结合临床和随访探讨，进一步完善诊断标准．43例恶性体腔积液的细胞染色体检查阳性率由常规法的51．2％提高到该法的90．7％，两法结果经γ~2检验（x~2＝16．29，P＜0．005），提示两法有显著性差异。经R带等显带分析，认为肺癌与3号染色体的短臂缺失［del（3）（p？）］有关，良性体腔积液中出现的超二倍体可能为非结核性潜在因素引起的。
The three-step low-speed centrifugation was used to purify the tumor cells of the malignant body effusion. The tumor cells were enriched as much as possible. At the same time, combined with clinical and follow-up to further improve the diagnostic criteria. The positive rate of chromosome chromosomal examination in 43 cases of malignant body effusion increased from 51.2% in the conventional method to 90.7% in the method. The results of the two methods were tested by gamma~2 (x~2=16.29, P<0). .005), suggesting significant differences between the two methods. After zonal banding analysis of the R band, it was suggested that lung cancer is related to the deletion of the short arm of chromosome 3 [del(3)(p?)]. The presence of hyperdiploids in benign fluid may be caused by non-tuberculous underlying factors. .