论文部分内容阅读
基于双通道表面等离子体子共振(surface plasmon resonance,SPR)传感器,分别采用直接法和金纳米粒子作为传感层的方法,通过检测赭曲霉毒素A(ochratoxin A,OTA)合成初期阶段表达的聚酮合成酶(polyketide synthase,PKS)基因的25个特异性碱基的寡核苷酸链,建立了一种高灵敏、间接检测OTA的新方法.同时考察了6-巯基己醇(6-mercapto-1-hexanol,MCH)作为封闭液对SPR响应信号的影响.结果表明,直接法的检测限为12.5 nmol/L,MCH的加入可使响应信号有所增强,使用金纳米粒子作为传感层的检测下限为0.25 nmol/L,与直接法相比较灵敏度提高了50倍,与以往使用金纳米粒子标记抗体或抗原相比,其作为传感层也能大大提高SPR检测灵敏度,且操作简单易行.
Based on the dual-channel surface plasmon resonance (SPR) sensor, direct method and gold nanoparticle were used as the sensing layer respectively to detect ochratoxin A (OTA) A 25-mer oligonucleotide chain of polyketide synthase (PKS) gene was developed to establish a highly sensitive and indirect method for the detection of OTA.At the same time, 6-mercapto -1 -hexanol, MCH) as a blocking solution on the SPR response signal.The results showed that the detection limit of the direct method was 12.5 nmol / L, the addition of MCH can enhance the response signal, the use of gold nanoparticles as the sensing layer Of the detection limit of 0.25 nmol / L, compared with the direct method of the sensitivity increased by 50 times, and the previous use of gold nanoparticles labeled antibody or antigen compared to the sensing layer can also greatly improve the SPR detection sensitivity, and the operation is simple and easy .