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目的 探讨肾母细胞瘤 16 q杂合性丢失 (LOH)的频率及其与选择性病理临床参数的联系。方法 抽提 32例肿瘤组织的DNA及其正常组织DNA ,用 16q2 4区域微卫星多肽标记 ,PCR方法对 16 q2 4多肽序列扩增 ,聚丙烯酰胺凝胶电泳 ,行LOH分析。其结果与患儿年龄、性别、临床分期、组织学类型和复发、转移、死亡作统计分析。结果 年龄大于 2 4个月患儿LOH 16q明显增多 (P<0 .0 5 ) ;LOH 16 q发生于临床Ⅱ期或Ⅲ期 ,明显高于Ⅰ期 (P <0 .0 5 ) ,而Ⅱ、Ⅲ期之间无明显差别。LOH 16 q与性别、肿瘤组织类型无关 ;LOH 16 q阳性患儿死亡率是LOH 16q阴性患儿的 4倍。 结论 LOH 16 q多见于小儿肾母细胞瘤 ,检测小儿肾母细胞瘤LOH 16q有益于高危人群的分类。
Objective To investigate the frequency of loss of heterozygosity (LOH) of 16 g nephroblastoma and its relationship with clinicopathological parameters of selective pathology. Methods The DNA and normal tissue DNA of 32 cases of tumor tissues were extracted and labeled with 16q2 4 microsatellite markers. The 16 q2 4 polypeptide was amplified by PCR and analyzed by polyacrylamide gel electrophoresis and LOH analysis. The results of children with age, gender, clinical stage, histological type and recurrence, metastasis, death for statistical analysis. Results LOH 16q was significantly increased in children older than 24 months (P <0.05). LOH 16 q occurred in stage Ⅱ or Ⅲ, which was significantly higher than that in stage Ⅰ (P0.05), while Ⅱ There was no significant difference between the three stages. LOH 16 q was not related to sex and tumor type; the mortality rate in LOH 16 q-positive children was four times that in LOH 16q-negative children. Conclusions LOH 16 q is more common in pediatric nephroblastoma. Detection of pediatric nephroblastoma LOH 16q is beneficial for the classification of high-risk groups.