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The mechanism of conjugal transfer of plasmids in Gram-negative and unicellular Gram-positive bacteria is commonly via a type Ⅳ secretion system (T4SS) [1].The genes encoding the T4S proteins are usually arranged in a single operon or a few operons.In Gram-negative Agrobacterium tumefaciens,the T4SS is encoded by the virB and virD operons (11 and 5 genes,respectively) [2].