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应用免疫荧光组织化学双重染色和三重染色技术 ,在激光共聚焦显微镜下观察了大鼠三叉神经中脑核 (Vme)内谷氨酸脱羧酶 (GAD)样、甘氨酸 (Gly)样和 P物质 (SP)样阳性终末与磷酸激活的谷氨酰胺酶(PAG)样阳性神经元之间的联系。结果显示 :1几乎所有的 Vme神经元均呈 PAG样免疫阳性 ,这些神经元绝大多数为大的假单极神经元。 2密集分布的 GAD样或 Gly样免疫阳性的神经终扣分别聚集于 PAG样阳性的 Vme神经元胞体周围 ,并与之形成密切接触。3 SP样免疫阳性终末与 PAG样阳性的 Vme神经元形成接触 ,且这些终末中有部分与 5-羟色胺 (5- HT)免疫活性物质共存。以上结果表明 :GABA能、Gly能和 SP能投射至 Vme的神经终末 ,可能在初级神经元即 Vme水平对谷氨酸介导的口面部本体觉信息的传递具有抑制性调控作用。
Immunofluorescence histochemistry double staining and triple staining were used to observe the changes of glutamate decarboxylase (GAD), glycine (Gly) and substance P (PgT) in the rat middle trigeminal nucleus (Vme) under confocal laser scanning microscope SP) -like positive terminals and phospho-activated glutaminase (PAG) -like neurons. The results showed that: 1 Almost all Vme neurons showed PAG-like immunopositivity, the vast majority of these neurons were large pseudopolar neurons. 2 Densely distributed GAD-like or Gly-like immunopositive neuro-terminalis respectively clustered around and in close contact with PAG-like positive Vomer neuronal soma. 3 SP-like immunopositive terminals are in contact with PAG-like positive Vme neurons and some of these terminals co-exist with serotonin (5-HT) immunoreactive substances. The above results indicate that GABA, Gly and SP can be projected to the terminal of Vme neurons, which may inhibit the transmission of glutamate-mediated oral and facial body sensory information at the primary neuron, Vme level.