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为探讨哮喘大鼠在连续激发不同阶段脑内小胶质细胞激活的时程及形态变化,本实验取健康雄性SD大鼠制备哮喘模型并诱发哮喘发作。48只SD大鼠随机分为对照组和实验组。对照组分为正常组和生理盐水组(n=8);实验组根据造模期间连续抗原激发的时间不同,分为连续激发3,7,14,21d组(n=8)。各组大鼠均进行呼吸功能检测、肺组织切片HE染色及脑组织抗OX-42免疫组织化学染色。结果显示:与对照组大鼠比较,实验组中各组大鼠呼吸功能各项指标均明显加重;肺组织病变程度逐渐增加;小胶质细胞由静息状态转变为激活状态,且数量明显增多。以上结果提示:哮喘大鼠脑内小胶质细胞被激活,在一定时间内小胶质细胞激活的程度随呼吸功能的降低及肺组织病变程度的增加而增加。
To investigate the duration and morphological changes of microglia activation in asthmatic rats at different stages of continuous stimulation, healthy male SD rats were used to prepare asthma models and induce asthma attacks. 48 SD rats were randomly divided into control group and experimental group. The control group was divided into normal group and normal saline group (n = 8). The experimental group was divided into continuous stimulation 3, 7, 14 and 21 days (n = 8) according to the time of continuous antigen challenge during the modeling. Respiratory function tests, lung tissue sections HE staining and brain tissue anti-OX-42 immunohistochemical staining. The results showed that compared with the control group, all indexes of respiratory function of rats in the experimental group were significantly increased; the degree of pulmonary lesions gradually increased; microglia changed from resting state to activated state, and the number was significantly increased . The above results suggest that microglia are activated in the brain of asthmatic rats, and the degree of microglial activation is increased with the decrease of respiratory function and the degree of pulmonary lesion in a certain period of time.