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本文通过正交设计试验,建立了一种改良的诱生及检测LICC细胞毒活性的培养体系。先将脾脏淋巴细胞(5×10~6/ml)在含有IL-2(0.5U/ml)、CCDF(50％V/V)RPMI1640培养液中预培养72小时,然后加入肿瘤细胞(5×10~4/ml),用~3H-TdR后标法检测LICC细胞毒活性,杀伤时间为72小时。
In this paper, through an orthogonal design experiment, an improved culture system of inducing LCCC cytotoxicity was established. Spleen lymphocytes (5 × 10 ~ 6 / ml) were preincubated for 72 hours in RPMI1640 medium containing IL-2 (0.5U / ml) and CCDF (50% V / V) 10 ~ 4 / ml). The cytotoxic activity of LICC was detected by ~ 3H-TdR post-labeling method. The killing time was 72 hours.