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目的：探讨抗氧化剂能否抑制内皮细胞表面的粘附分子表达和内皮细胞与白细胞粘附，以及抗氧化剂调节ＮＦ－κΒ的活性是否通过一种对氧化还原敏感的机制。方法：在一种液体流动小室中进行细胞粘附实验；抗氧化剂的抗氧化活性用２’－７’－二氯荧光素双醋酸盐（ＤＣＦＨ－ＤＡ）染色血管内皮细胞经流式细胞仪测量；内皮细胞ＮＦ－κＢ的活性用电泳迁移率分析测定。结果：ＰＤＴＣ和ｃｈｒｙｓｉｎ抑制ＨＬ６０往ＴＮＦα刺激的ＨＵＶＥＣ上粘附，ｐｒｏｂｕｃｏｌ和ＤＣＩ则没有作用。ＤＣＩ和ｃｈｒｙｓｉｎ抑制内皮细胞过氧化氢的形成，而ＰＤＴＣ和ｐｒｏｂｕｃｏｌ无效。只有ＰＤＴＣ对ＮＦ－κＢ的活性有抑制作用，其他抗氧化剂对ＮＦ－κＢ的活性没有影响。结论：（１）ＰＤＴＣ对ＮＦ－κＢ的抑制作用与它的抗氧化特性无关；（２）ＤＣＩ和ｃｈｒｙｓｉｎ对ＲＯＩ的清除作用及其对白细胞粘附的抑制作用与抑制ＮＦ－κＢ活性无关。
OBJECTIVE: To investigate whether antioxidants inhibit the expression of adhesion molecules on endothelial cells and the adhesion of endothelial cells to leukocytes, and whether antioxidant activity of NF-κB is mediated by a redox-sensitive mechanism. METHODS: Cell adhesion experiments were performed in a fluid flow chamber; antioxidant activity of anti-oxidant vascular endothelial cells were stained with 2’-7’-dichlorofluorescein diacetate (DCFH-DA) by flow cytometry The activity of NF-κB in endothelial cells was determined by electrophoretic mobility shift assay. RESULTS: PDTC and chrysin inhibited HL60 adhesion to TNFα-stimulated HUVECs, but probucol and DCI had no effect. DCI and chrysin inhibit endothelial cell hydrogen peroxide formation, and PDTC and probucol ineffective. Only PDTC has an inhibitory effect on the activity of NF-κB, and other antioxidants have no effect on the activity of NF-κB. CONCLUSION: (1) The inhibitory effect of PDTC on NF-κB has nothing to do with its anti-oxidative properties. (2) The scavenging effect of DCI and chrysin on ROI and its inhibitory effect on leukocyte adhesion have nothing to do with the inhibition of NF-κB activity.