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目的评估激活素A(ACT)以及其拮抗剂卵泡抑素(follistatin),对白介素-6(IL-6)、白介素-8(IL-8)和血管内皮生长因子(VEGF)等在上述2种细胞内的表达与释放的影响。方法来源于患有子宫内膜异位症的女性(n=10)和正常对照女性(n=10)的人表皮间质细胞,以不同剂量ACT处理或ACT合并卵泡抑素双处理。实时荧光定量PCR检测IL-6、IL-8、VEGF表达,ELISA法检测蛋白水平。结果患病组细胞中IL-6分泌基线水平是正常组的2.04倍,IL-8的分泌基线水平是正常组的6.35倍(P<0.05),而ACT处理则显著上调正常组中IL-8的表达与分泌以及VEGF的分泌水平,并显著下调患病组中IL-6和VEGF的分泌水平,而且ACT的作用效果可被卵泡抑素抵消(P<0.05)。结论 ACT能够调控体外培养的表皮间质细胞中IL-6、IL-8和VEGF的表达与分泌,而该机制似乎在患有子宫内膜异位女性的细胞中存在缺陷。
Objective To evaluate the effects of activin A (ACT) and its antagonist follistatin on interleukin-6 (IL-6), interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF) Effect of intracellular expression and release. Methods Human epidermal mesenchymal cells from women with endometriosis (n = 10) and normal controls (n = 10) were treated with different doses of ACT or with ACT combined with follistatin. The expression of IL-6, IL-8 and VEGF were detected by real-time fluorescence quantitative PCR and the protein level by ELISA. Results The baseline level of IL-6 secretion was 2.04 times of that of the normal control group and the baseline level of IL-8 secretion was 6.35-fold higher than that of the normal control group (P <0.05), but the level of IL-8 (P <0.05). The level of IL-6 and VEGF secretion in the patients was significantly lower than that in the control group (P <0.05). Conclusion ACT regulates the expression and secretion of IL-6, IL-8 and VEGF in epidermal stromal cells cultured in vitro, and this mechanism seems to be deficient in cells with endometriosis.