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目的探讨叔丁基对苯二酚(tert-butylhydroquinone,tBHQ)对亚砷酸钠(NaAsO2)致Changliver细胞毒性的影响。方法Changliver细胞培养48h后分别以20、40、60和80μmol/L的NaAsO2染毒24和48h,作为NaAsO2单独作用组。以5和20μmol/L的tBHQ预处理Chang liver细胞24h,以40和60μmol/L的NaAsO2染毒24和48h,作为tBHQ预处理组;对照组处理同NaAsO2单独作用组。每个浓度设3个复孔。用Alamar Blue法检测细胞活力。结果NaAsO2单独作用24和48h组Alamar Blue还原率显著下降,与对照组比较,差异有统计学意义(P<0.05);且NaAsO2单独作用48h组的Alamar Blue还原率均显著低于24h组,差异有统计学意义(P<0.01)。5μmol/L的tBHQ预处理组与对应的60μmol/LNaAsO2单独作用24h组相比,显著提高了Alamar Blue还原率(P<0.01);20μmol/L的tBHQ预处理组的AlamarBlue还原率均显著高于相对应NaAsO2单独作用24h组(P<0.05)。5μmol/L的tBHQ预处理组的Alamar Blue还原率均显著高于相对应NaAsO2单独作用48h组(P<0.01);20μmol/L的tBHQ预处理组与对应的40μmol/LNaAsO2单独作用48h组相比,显著提高了Alamar Blue还原率(P<0.01)。结论tBHQ能够降低NaAsO2致Changliver细胞的毒性,增强细胞对NaAsO2毒性的抵抗能力。
Objective To investigate the effects of tert-butylhydroquinone (tBHQ) on the cytotoxicity of Changliver induced by sodium arsenite (NaAsO2). Methods After 48 hours of culture, Changliver cells were exposed to 20, 40, 60 and 80μmol / L NaAsO2 for 24 and 48 hours, respectively. Chang liver cells were pretreated with tBHQ of 5 and 20 μmol / L for 24 h and treated with 40 and 60 μmol / L of NaAsO2 for 24 and 48 h respectively, which were treated with tBHQ pretreatment group and control group treated with NaAsO2 alone. Set three holes for each concentration. Cell viability was measured by Alamar Blue assay. Results Alamar Blue reduction rate decreased significantly in NaAsO2 treated group for 24 and 48h (P <0.05), and Alamar Blue reduction rate in NaAsO2 treated group for 48h was significantly lower than that in 24h group There was statistical significance (P <0.01). The Alamar Blue reduction rate was significantly increased (P <0.01) compared with the control group treated with 60μmol / LNaAsO2 for 5μmol / L tbHQ pretreatment alone, and the AlamarBlue reduction rate was significantly higher in the 20μmol / L tBHQ pretreatment group Corresponding NaAsO2 alone 24h group (P <0.05). Alamar Blue reduction rate in 5μmol / L tBHQ pretreatment group was significantly higher than that in NaAsO2 alone 48h group (P <0.01); 20μmol / L tBHQ pretreatment group and corresponding 40μmol / LNaAsO2 alone 48h group , Significantly increased the Alamar Blue reduction rate (P <0.01). Conclusion tBHQ can reduce the toxicity of NaAsO2 to Changliver cells and enhance the resistance of cells to NaAsO2 toxicity.