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对重庆地区汉族群体200例无关个体进行Amelogenin及9个STR基因座群体遗传学调查，并对人血液（痕）、精斑、组织、染色的细胞涂片、毛发、牙齿及骨骼组织进行基因型测定，并用于法科学检查。采用Chelex或酚-氯仿提取DNA荧光标记复合扩增法对Amelogenin及9个STR基因座，即D3S1358、VWA、FGA、TH01．TPOX、CSF1PO、DSS818、D135317、D75820进行复合扩增，扩增产物由毛细管电泳进行分离和荧光检测。9个STR基因座分别发现6、7、17、7、5、8、7、7、7个等位基因，其最高的基因频率分别是0．3900、0．2480、0．1650、0．5380、0．5280、0．4500、0．3140、0．3150、0．4000。发现的基因型数分别是16、25、71、20、12、22、26、22、26个。基因型频率均符合Hardy－Weinberg平衡，未发现基因连锁。9个STR基因座的DP值分别是0．8610、0．9220、0．9750、0．8020、0．7730、0．8780、0．9210、0．9140、0．8990，累计DP值是0．9999；非父排除率分别是0．5990、0．5450、0．7650、0．2950、0．3620、0．4520、0．6840、0．6660、0．5180，累积非父排除率是0．9995；杂合度分别是0．8000、0．7700、0．8850、0．6030、0．6550、0．7150、0．8440、0．8350、0．7550，累积杂合度是0．9999。证明这些基因座均适用于重庆
Amelogenin and nine STR loci were investigated in 200 unrelated Chinese Han population in Chongqing. The genotypes of human blood (scar), seminal plasma, tissue, stained cell smears, hair, teeth and bone were genotyped Determination, and for scientific research. Amelogenin and 9 STR loci, namely D3S1358, VWA, FGA and TH01, were amplified by Chelex or phenol-chloroform extraction DNA fluorescent labeling multiplex amplification. TPOX, CSF1PO, DSS818, D135317, D75820 for amplification. The amplified products were separated by capillary electrophoresis and detected by fluorescence. The 9 STR loci found 6, 7, 17, 7, 5, 8, 7, 7 and 7 alleles, respectively. The highest frequency of these alleles was 0.3900, 0.2480 and 0.1650 respectively. 5380, 0.5280, 0.4500, 0.3140, 0.3150, 0.4000. The number of found genotypes were 16,25,71,20,12,22,26,22,26. Genotype frequencies were in line with the Hardy-Weinberg equilibrium, found no genetic linkage. The DP values of the nine STR loci were 0.8610, 0.9220, 0.9750, 0.8020, 0.7730, 0.8780, 0.9210, 0.9140, 0.8990 respectively. 0.9999; non-parent exclusion rates were 0.5990,0.5450,0.7650,0.2950,0.3620,0.4520,0.6840,0.6660,0.5180 cumulative non-parent exclusion The rate was 0.9995; the heterozygosity was 0.8000,0.7700,0.8850,0.6030,0.6550,0.7150,0.8440,0.8350,0.7550, the cumulative heterozygosity was 0.9999. Prove that these loci are suitable for Chongqing