论文部分内容阅读
本实验采用免疫组化及显微分光扫描测定法,观察了浸水拘束性应激刺激对大鼠胃窦及十二指肠5-羟色胺(5-HT)细胞内5—HT含量的影响.实验结果表明,浸水拘束性刺激8 h后,实验组5只大鼠的瘤胃全部发生溃疡,但十二指肠未见溃疡发生,正常对照5只大鼠均无溃疡发生.对应激组大鼠的胃窦切片进行5-HT细胞扫描,发现5只大鼠的胃窦5—HT细胞内5—HT明显增加.应激组n=100(细胞数)为0.506±0.058 AU(为随意单位值),明显高于正常对照组0.460±O.070 AU P<0.01.相反,应激组大鼠十二指肠内5-HT细胞的5-HT含量明显降低.对照组为0.380±0.007 AU,应激组为0.350±0.040 AU,P<0.01.此结果提示胃窦内源性5—HT可能与应激性溃疡的形成有关.
In this experiment, immunohistochemistry and microscopic spectroscopic scanning assays were used to observe the effects of water-soaked stress stimuli on the contents of 5-HT in 5-hydroxytryptamine (5-HT) cells in gastric antrum and duodenum of rats. The results showed that after 8 hours of restrictive water-soaked stimulation, all five rats in the experimental group had ulcers in the rumen, but no duodenal ulcers occurred, and no ulcers occurred in the normal control rats. The gastric antrum sections were subjected to 5-HT cell scan and 5-HT in the gastric antrum 5-HT was significantly increased in five rats. The stress group n=100 (cell number) was 0.506±0.058 AU (random unit value). , significantly higher than the normal control group 0.460 ± O.070 AU P <0.01. On the contrary, the 5-HT content of 5-HT cells in the duodenum of the stress group rats decreased significantly. The control group was 0.380 ± 0.007 AU, should The stress group was 0.350±0.040 AU,P<0.01. This result suggested that the endogenous 5-HT in antrum may be related to the formation of stress ulcer.