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为了开展恶性疟疾的防治研究,需要通过体外培养获得大量的恶性疟原虫配子体,以便为研究恶性疟原虫的有性期创造实验条件。一、培养方法及结果 Row(1929)首先用非常简单的方法,培养出未成熟的恶性疟原虫配子体。自从Trager(1975)连续培养恶性疟原虫红内期获得成功后,为恶性疟虫原配子体体外培养提供了技术条件。Smalley(1976)从27例6个月到6岁的恶性疟患者静脉取血,用199培养基洗涤1次,再用下列培养基培养:199培养基含葡
In order to carry out research on the prevention and treatment of malaria, a large number of Plasmodium falciparum gametophytes need to be obtained through in vitro culture so as to create experimental conditions for studying the sexual period of Plasmodium falciparum. First, the training methods and results Row (1929) first with a very simple method to cultivate immature Plasmodium falciparum gametophyte. Since Trager (1975) succeeded in culturing Plasmodium falciparum in a continuous manner, it provided the technical conditions for the in vitro culture of progenad of Plasmodium falciparum. Smalley (1976) took venous blood from 27 patients with falciparum malaria, 6 months to 6 years old, washed once with 199 medium, and then with the following medium: 199 medium containing Portuguese