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以王米的3个自交系及其配制的3个单交种的5种不同外植体诱导愈伤组织。7～8叶期的幼茎切段和授粉后22天的幼胚表现有较高的出愈率。由幼芽切段培养的愈伤组织经多次固体继代和筛选呈浅黄色颗粒状,且在其液体悬浮培养中观察到少数细胞的一次分裂相,并对收集到的单细胞和悬浮愈伤组织进行电镜扫描观察。在愈伤组织继代培养中,适宜的2,4-D 浓度为2.5～3.0mg/l。愈伤组织生长快慢与接种时愈伤组织初始体积大小有关。其直径≤0.5厘米的生长较慢。愈伤组织在两种分化培养基上或硬化或形成大量的根,未能获得再生植株。
The callus was induced by 5 different explants from 3 inbred lines of Wangmi and 3 single crosses. Young stem segments from 7 to 8 leaf stages and immature embryos from 22 days after pollination showed higher rates of healing. The callus cultured by cuttings of young shoots was repeatedly subcultured and screened to be light yellow granules, and the primary cell division phase was observed in its liquid suspension culture. The collected single cells and suspended callus Injured tissue scanning electron microscopy. In callus subculture, the suitable 2,4-D concentration is 2.5 ~ 3.0mg / l. The speed of callus growth is related to the initial callus volume at the time of inoculation. Its diameter ≤ 0.5 cm slower growth. Callus hardens or forms large numbers of roots on both differentiation media, failing to obtain regenerated plants.