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里氏木霉（ＴｒｉｃｈｄｅｒｍａｒｅｓｅｉＲｕｔＣ３０）以经蒸汽爆破预处理的玉米秆为底物制备纤维素酶，产酶ｐＨ值维持在５．０左右，底物浓度为３．７５％（玉米秆，干基）时，产酶３天，滤纸酶活力和纤维二糖酶活力达１．７６ＩＵ／ｍＬ和０．３８ＩＵ／ｍＬ，纤维二糖酶活力高，酶水解得率达８０．２％；在试验培养基中添加纤维二糖对纤维素酶的合成具有抑制作用，培养基中添加纤维二糖浓度在０．２５～１．５０ｇ／Ｌ时，滤纸酶活力和纤维二糖酶活力同时下降３０％～４０％；淀粉水解液对纤维毒酶合成的影响是由葡萄糖的抑制和复合糖的诱导共同作用的结果，在用本研究培养基制备纤维酶过程中添加淀粉水解液，从经济角度来说是不适宜的。
Trichoderma reesei (TrichdermareseiRutC30) by steam explosion pretreatment of corn stalks as substrate cellulase enzyme production pH was maintained at about 5.0, the substrate concentration of 3.75% (corn stalks, dry basis) The enzyme activity and cellobiase activity reached 1.76 IU / mL and 0.38 IU / mL, respectively. The cellobiase activity was high and the rate of enzymatic hydrolysis was 80.2% The addition of cellobiose inhibited the synthesis of cellulase. When the cellobiose concentration was 0.25 ~ 1.50g / L, the activity of filter paper and the activity of cellobiase decreased by 30% -40 %; The effect of starch hydrolyzate on the synthesis of fiber toxins is the result of the synergism of the inhibition of glucose and the induction of complex sugars. Adding starch hydrolyzate during the preparation of cellulases in this study medium is economically not suitable.