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目的:研究天然牛磺酸抑制大鼠肝硬化门静脉高压症的疗效及可能机制。方法:采用复合因素法制作肝硬化模型,60只Wistar大鼠随机分为正常对照组、模型组、天然牛磺酸治疗组,每组20只。检测治疗前后各组门静脉压力(PVP)、门静脉血流量(PVF),平均动脉压(MAP)、心率(HR),肝功能,血清Ⅳ-C、PCⅢ、HA、LN含量,肝脏组织学,采用硝酸还原酶法测定一氧化氮(NO)含量,化学比色法测定一氧化氮合酶(NOS)活性,放射免疫法检测环鸟苷酸(cCMP)含量。结果:天然牛磺酸组PVP、PVF降低,MAP升高,HR减慢,与模型组相比差异有显著意义(P<0.05);ALT降低,ALB升高,与模型组相比差异有显著意义(P<0.01);TBIL无明显变化,与模型组相比差异无显著意义(P>0.05);Ⅳ-C、PCⅢ、HA、LN含量均显著降低,与模型组相比差异有显著意义(P<0.01);NO、cCMP含量及NOS活性也均有不同程度的降低,与模型组相比差异有显著意义(P<0.05);同时肝脏组织结构有所改善。结论:天然牛磺酸能够有效地降低大鼠肝硬化门静脉压力,对肝硬化门静脉高压的形成机制有“双向”调节作用,具有良好的临床开发和应用前景。
Objective: To study the curative effect and possible mechanism of natural taurine on cirrhotic portal hypertension in rats. Methods: The liver cirrhosis model was made by the composite factor method. Sixty Wistar rats were randomly divided into normal control group, model group and natural taurine treatment group, with 20 rats in each group. The levels of PVP, PVF, MAP, HR, liver function, serum IV-C, PCⅢ, HA, LN in each group were measured before and after treatment. Nitric acid reductase method was used to determine nitric oxide (NO) content, nitric oxide synthase (NOS) activity was determined by chemical colorimetric method, and cyclic guanosine monophosphate (cCMP) content was detected by radioimmunoassay. Results: Compared with the model group, the changes of PVP, PVF, MAP and HR in natural taurine group were significant (P <0.05); ALT decreased and ALB increased significantly compared with model group (P <0.01). There was no significant difference in TBIL between the model group and the model group (P> 0.05). The contents of Ⅳ-C, PCⅢ, HA and LN were significantly decreased (P <0.01). NO, cCMP content and NOS activity also decreased to some extents, which was significantly different from that of the model group (P <0.05). At the same time, the liver tissue structure was improved. CONCLUSION: Natural taurine can effectively reduce the portal vein pressure in rats with cirrhosis and regulate the formation mechanism of portal hypertension in cirrhosis. It has good clinical development and application prospects.