Using the nematode Caenorhabditis elegans as a model animal for assessing the toxicity induced by mi

• 作 者: LI Yunhui WANG Yang YIN Lihong
• 机 构: School of Public Health, Southeast University, Nanjing 210009, China“,”Key Laboratory of Development
• 出 处: 环境科学学报（英文版）
• 发表日期: 2009年3期
• 关键词: microcystin-LR toxicity assessment brood size stress response Caenorhabditis ele

Among more than 75 variants of microcystin (MC), microcystin-LR (MC-LR) is one of the most common toxins. In this study, the feasibility of using Caenorhabditis elegans to evaluate MC-LR toxicity was studied. C. elegans was treated with MC-LR at different concentrations ranging from 0.1 to 80 μg/L. The results showed that MC-LR could reduce lifespan, delay development, lengthen generation times, decrease brood sizes, suppress locomotion behaviors, and decreases hsp-16-2-gfp expression. The endpoints of generation time, brood size, and percentage of the population expressing hsp16-2-gfp were very sensitive to 1.0 μg/L of MC-LR, and would be more useful for the evaluation of MC-LR toxicity. Furthermore, the tissue-specific hsp16-2-gfp expressions were investigated in MC-LR-exposed animals, and the nervous system and intestine were primarily effected by MC-LR. Therefore, the generation time, brood size, and hsp16-2-gfp expression in C. elegans can be explored to serve as valuable endpoints for evaluating the potential toxicity from MC-LR exposure.