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目的:研究小鼠腹腔巨噬细胞体外对环孢菌素A胶体亚微粒的摄取。方法:制备[~3H]-环孢菌素A纳米球、纳米囊和微乳三种胶体亚微粒,以小鼠腹腔巨噬细胞为体外细胞模型,研究小鼠腹腔巨噬细胞体外对环孢菌素A胶体亚微粒的摄取。结果:纳米球可使巨噬细胞对环孢菌素A的摄取达对照溶液的20倍,而纳米囊和微乳则使巨噬细胞对环孢菌素A的摄取明显减少。在胶体亚微粒的表面进行表面活性剂修饰和血浆蛋白吸附对巨噬细胞的摄取有明显影响。结论:将环孢菌素A包封于胶体亚微粒中能改变其对巨噬细胞的靶向作用。
Objective: To study the uptake of cyclosporine A colloidal submicrospheres in mouse peritoneal macrophages in vitro. METHODS: Three kinds of colloidal submicrospheres of [~ 3H] -cyclosporin A nanocapsules, nanocapsules and microemulsions were prepared. The mouse peritoneal macrophages were used as in vitro cell models to study the effect of peritoneal macrophages Ingestion of mycin A colloidal submicron. Results: Nanospheres induced 20-fold uptake of cyclosporin A by macrophages, whereas nanocapsules and microemulsions significantly reduced the uptake of cyclosporin A by macrophages. Surfactant modification and plasma protein adsorption on the surface of colloidal submicrospheres have a significant effect on macrophage uptake. CONCLUSION: Cyclosporin A encapsulation in colloidal submicrospheres alters its targeting effect on macrophages.