论文部分内容阅读
目的探讨TGF-β/smad蛋白在大鼠高氧肺损伤中的表达及意义,探求高氧肺损伤的原因以及TGF-β/smad蛋白的表达及其作用。方法 60只10 d日龄的大鼠,随机分为实验组和对照组,实验组大鼠让其处于95%氧气的高氧环境下暴露7 d,而对照组在正常的空气环境下暴露7 d,观察检测大鼠的肺情况,TGF-β/smad蛋白,肺组织LKB1、TGF-β1、psmad2蛋白的表达和分布,分析蛋白的表达和意义。结果与对照组比较,实验组的肺组织LKB1、TGF-β1、psmad2蛋白下降明显,呈高表达,实验组肺组织中LKB1与TGF-β1蛋白表达呈正相关(r=0.474,P=0.04),TGF-β1与psmad2蛋白表达呈正相关(r=0.491,P=0.033)结论 TGF-β/smad蛋白可以作为判断高氧的肺损伤的损害程度和判预后的一种重要指标,并且受到信号分子的调控,在高氧的情况下,可影响肺功能的正常情况,并对肺部产生损伤。
Objective To investigate the expression and significance of TGF-β / smad protein in hyperoxia-induced lung injury in rats and explore the causes of hyperoxia-induced lung injury and the expression of TGF-β / smad protein. Methods Sixty (10) day-old rats were randomly divided into experimental group and control group. The rats in experimental group were exposed to 95% oxygen for 7 days under aerobic conditions, while the control group were exposed to normal air for 7 days d. The expression and distribution of lung, TGF-β / smad protein, LKB1, TGF-β1, psmad2 protein in lung tissue were observed and the protein expression and significance were analyzed. Results Compared with the control group, the expression of LKB1, TGF-β1 and psmad2 in the experimental group decreased significantly and the expression of LKB1 and TGF-β1 protein in the experimental group was positively correlated (r = 0.474, P = 0.04) TGF-β1 and psmad2 protein expression was positively correlated (r = 0.491, P = 0.033) Conclusion TGF-β / smad protein can be used as a judge to determine the extent of damage and hyperoxia induced lung injury after an important indicator, and by signaling molecules Regulation, in the case of hyperoxia, can affect normal lung function and cause damage to the lungs.