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为明确靶标害虫对Bt蛋白的抗性及对不同类型Bt蛋白的交互抗性,采用生物测定法进行了二化螟Chilo suppressalis(Walker)敏感品系和Cry1Ac汰选品系在Cry1Ac毒饲料上的时间-死亡率反应、对不同Bt杀虫蛋白的敏感性及不同Bt蛋白复配组合对二化螟毒力效果的研究。结果表明,二化螟Cry1Ac汰选品系已对36.67μg/mL(LC_(50))的Cry1Ac杀虫蛋白产生了一定的适应性,但对308.69μg/mL(LC90)的Cry1Ac蛋白反应仍较敏感。Cry1Ac敏感品系和汰选品系对Cry1Ab蛋白的LC_(50)分别为1.40μg/mL和3.86μg/mL,二者差异显著,但对Cry1Ca(1.63μg/mL和1.73μg/mL)或Cry2Aa(127.48μg/mL和144.50μg/mL)的LC_(50)差异不显著,即Cry1Ac汰选品系与Cry1Ab存在明显的交互抗性,但与Cry1Ca和Cry2Aa不存在交互抗性。在不同Bt蛋白复配组合中(1∶1复配),Cry1Ab+Cry1Ca、Cry1Ab+Cry2Aa和Cry1Ca+Cry2Aa增效作用最为显著。表明抗虫基因cry1Ca和cry2Aa可作为双价转基因抗虫水稻研发的候选基因。
In order to clarify the resistance of target pests to Bt protein and cross resistance to different types of Bt proteins, the bioassay was used to determine the time-response of Chilo suppressalis (Walker) and CrylAc- Mortality, sensitivity to different Bt insecticidal proteins and the effect of different Bt protein combinations on the virulence of the stem borer. The results showed that the Cry1Ac knockout strain of Chilo suppressalis had some adaptability to Cry1Ac insecticidal protein of 36.67μg / mL (LC_ (50)), but still sensitive to Cry1Ac protein of 308.69μg / mL (LC90) . The LC 50 of Cry1Ac sensitive strains and eliminated strains were 1.40μg / mL and 3.86μg / mL for Cry1Ab protein, respectively, but significant difference was observed for Cry1Ca (1.63μg / mL and 1.73μg / mL) or Cry2Aa (127.48 μg / mL and 144.50μg / mL). There was no significant cross-resistance between Cry1Ac and Cry1Ab, but there was no cross-resistance with Cry1Ca and Cry2Aa. The synergistic effects of Cry1Ab + Cry1Ca, Cry1Ab + Cry2Aa and Cry1Ca + Cry2Aa were the most significant among different Bt protein combinations (1: 1 combination). The results showed that the cry1Ca and cry2Aa genes could be used as candidate genes for bivalent transgenic insect-resistant rice.