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目的:采用正交试验和大孔树脂吸附法优化杠板归总黄酮提取和纯化工艺条件,为其开发和利用提供参考。方法:首先以提取时间、次数、温度和料液比为考察因素,采用正交试验优选提取工艺;接着以静态吸附容量、动态吸附率为考察指标,从4种型号大孔树脂中筛选出1种,并对其吸附和解吸条件进行探究。结果:杠板归总黄酮最佳提取工艺为提取2次,每次100 min,温度80℃,料液比1:12;HPD-100型大孔树脂适宜杠板归总黄酮的纯化,其纯化工艺为上样液浓度2.5 g/L,上样速率2 BV(柱体积)/h,上样液体积2.5 BV,用70%乙醇以2 BV/h流速洗脱,在此工艺条件下,吸附的总黄酮纯度55.4%,解吸得率83.2%。结论:该提取和纯化工艺稳定、简单、可行,可用于工业生产。
OBJECTIVE: To optimize the conditions of extraction and purification of total flavonoids from Plectranthus annuus by orthogonal test and macroporous resin adsorption method, and provide reference for its development and utilization. Methods: Firstly, the extraction time, times, temperature and the ratio of solid to liquid were taken as the investigation factors. The orthogonal experiment was adopted to optimize the extraction process. Then, the static adsorption capacity and the dynamic adsorption rate were taken as the investigation indexes. Species, and its adsorption and desorption conditions to explore. Results: The optimal extraction process of total flavonoids from Phellodendron chinense was extraction time was 100 min, temperature was 80 ℃, and the ratio of material to liquid was 1:12. HPD-100 macroporous resin was suitable for the purification of total flavonoids from plywood, The process consisted of a sample volume of 2.5 g / L, a loading rate of 2 BV (column volume) / h, a sample volume of 2.5 BV and a flow rate of 2 BV / h with 70% ethanol. Under these conditions, The total flavonoids purity of 55.4%, desorption rate of 83.2%. Conclusion: The extraction and purification process is stable, simple and feasible and can be used in industrial production.