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目的比较亚硒酸钠(Na_2SeO_3)、硒代蛋氨酸(SeMet)和甲基硒代半胱氨酸(MeSeCys)3种硒化合物在HepG2和Hela细胞中代谢生成硒蛋白P(SEPP)和谷胱甘肽过氧化物酶(GPx)的差异。方法将培养好的细胞分为对照组、Na_2SeO_3组、SeMet组和MeSeCys组。加入0.01μmol/L和0.1μmol/L的硒化合物作用48 h和72 h,收集细胞培养上清液和裂解液。采用酶联免疫吸附试验(ELISA)双抗体夹心法对上清中的SEPP和裂解液中的GPx进行定量检测。结果与对照组比较,0.1μmol/L SeMet和MeSeCys处理的Hela细胞生成的SEPP和GPx浓度显著升高(P<0.05)。与Hela细胞比较,0.1μmol/L 3种硒化合物处理的HepG2细胞生成的SEPP和GPx浓度均显著升高(P<0.05)。结论硒化合物在肝癌细胞HepG2中代谢生成硒蛋白的效应比在宫颈癌细胞Hela中更明显。
Objective To compare the metabolism of selenoprotein P (SEPP) and glutathione (GSH) in HepG2 and Hela cells by comparing three selenium compounds of sodium selenite, SeMet and MeSeCys, Differences in peptide peroxidase (GPx). Methods The cultured cells were divided into control group, Na_2SeO_3 group, SeMet group and MeSeCys group. After adding 0.01μmol / L and 0.1μmol / L selenium compounds for 48 h and 72 h, the cell culture supernatant and lysate were collected. Quantitative detection of SEx in the supernatant and GPx in the lysate was performed by enzyme-linked immunosorbent assay (ELISA) double antibody sandwich method. Results Compared with the control group, the concentrations of SEPP and GPx in Hela cells treated with 0.1μmol / L SeMet and MeSeCys were significantly increased (P <0.05). Compared with Hela cells, the concentrations of SEPP and GPx in HepG2 cells treated with 0.1μmol / L selenium compound were significantly increased (P <0.05). Conclusion The effect of selenium on the metabolism of selenoprotein in HepG2 cells is more obvious than that in Hela cells.