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目的探讨早期丰富环境对脑白质损害大鼠海马生长相关蛋白43(GAP-43)表达的影响。方法 2日龄新生大鼠29只,随机分为正常对照组(n=9)、干预组(n=10)及非干预组(n=10),后2组结扎右侧颈总动脉,术后休息2h,置密封舱中,吸入氧体积分数为60mL.L-1的氮氧混合气,制作新生大鼠脑白质损伤(WMD)模型。干预组采用早期触摸和丰富环境进行干预。早期触摸从损伤后第4天开始,连续实施7d。丰富环境干预从损伤后第11天开始,到第30天结束,总干预时间为27d。余2组正常饲养。干预结束后进行感觉运动功能检测,以判断干预效果。干预后第7天和第27天将大鼠处死,取其脑组织应用Westernblot方法检测海马GAP-43的表达。结果干预组感觉运动功能较非干预组显著改善(P<0.01)。非干预组和干预组海马GAP-43的表达在干预后7d时均高于正常对照组,在27d时干预组高于非干预组和正常对照组(Pa<0.01)。结论早期丰富环境可以上调脑白质损害大鼠海马GAP-43的表达,可能是其改善脑功能的机制之一。
Objective To investigate the effect of early rich environment on the expression of GAP-43 in rats with white matter lesion. Methods 29 neonatal rats of 2 days old were randomly divided into normal control group (n = 9), intervention group (n = 10) and non-intervention group (n = 10) After rest 2h, placed in a sealed chamber, inhaled oxygen volume fraction of 60mL.L-1 of nitrogen and oxygen mixture, the preparation of newborn rat white matter damage (WMD) model. The intervention group used early touch and rich environment to intervene. Early touch from the first 4 days after injury, continuous implementation of 7d. Enriched environmental intervention began on the 11th day after injury and ended on the 30th day with a total intervention time of 27 days. I 2 groups of normal breeding. Sensory motor function testing after intervention to determine the intervention effect. The rats were sacrificed on the 7th and 27th days after the intervention, and the brain tissue was used to detect the expression of GAP-43 in the hippocampus by Western blot. Results Compared with the non-intervention group, the sensory motor function of the intervention group was significantly improved (P <0.01). The expression of GAP-43 in hippocampus of non-intervention group and intervention group was higher than that of normal control group on the 7th day after intervention, and higher on the 27th day than that of the non-intervention group and the normal control group (Pa <0.01). Conclusion Early enrichment can up-regulate the expression of GAP-43 in the hippocampus of rats with white matter lesion, which may be one of the mechanisms of improving brain function.