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目的 :探讨肠缺血 /再灌注时肿瘤坏死因子 α(tumornecrosisfactor α ,TNF α)的来源及作用。方法 :建立幼鼠肠缺血 /再灌注动物模型 ,利用放射免疫法检测门静脉和外周血血清中TNF α表达及对肠组织进行形态学观察。结果 :缺血 30min组肠粘膜轻度受损 ,缺血 30min /再灌注 30min组、缺血 30min/再灌注 6 0min组、缺血 30min/再灌注 90min组肠粘膜中度受损 ,缺血 12 0min组肠粘膜重度受损。肠缺血 30min组门静脉血清TNF α开始升高为 (2 .0 6± 0 .5 2 )ng/ml,缺血 30min/再灌注 30min组达峰值为 (2 .96± 0 .4 5 )ng/ml,二者皆明显高于对照组 (P <0 .0 5 ) ;缺血 30min/再灌注 6 0min组、缺血 30min/再灌注 90min组时下降 ;肠缺血 30min/再灌注 30min组外周血清TNF α也升高为 (2 .11± 0 .2 9)ng/ml(P <0 .0 5 )。且缺血 30min组、缺血 30min/再灌注 30min组、缺血 12 0min组门静脉TNF α水平显著高于外周血 (P <0 .0 5 )。结论 :幼鼠肠缺血 /再灌注后TNF α在肠、肝内均有产生 ,但门静脉血清TNF α较外周血血清升高明显 ,因此推论幼鼠肠缺血 /再灌注损伤TNF α可能主要来源于肠组织 ,并且介导幼鼠肠缺血 /再灌注损伤的病理过程
Objective: To investigate the origin and function of tumor necrosis factor α (TNFα) during intestinal ischemia / reperfusion. Methods: The animal model of intestinal ischemia / reperfusion was established in young rats. The expression of TNFα in portal vein and peripheral blood was detected by radioimmunoassay and morphological changes of intestinal tissue were observed. Results: Intestinal mucosa was mildly damaged 30 min after ischemia / 30 min / reperfusion 30 min, ischemia 30 min / reperfusion 60 min, ischemia 30 min / reperfusion 90 min, 0min group, severe damage to intestinal mucosa. The portal vein TNF-α began to increase to (2.06 ± 0.52) ng / ml after 30min ischemia, and peaked at 30min after ischemia / 30min reperfusion (2.96 ± 0.45ng) / ml, both of which were significantly higher than that of the control group (P <0.05); the group of ischemia 30 min / reperfusion 60 min, the group of ischemia 30 min / reperfusion 90 min decreased, the group of intestinal ischemia 30 min / reperfusion 30 min Peripheral serum TNFα also increased to (2.11 ± 0.29) ng / ml (P <0.05). The level of TNFαin the portal vein was significantly higher than that in the peripheral blood (P <0.05) at 30 min ischemia, 30 min ischemia / 30 min reperfusion. CONCLUSIONS: TNFα is produced in the intestine and liver after intestinal ischemia-reperfusion in young rats, but the serum TNF-α level in portal vein is significantly higher than that in peripheral blood. Therefore, it is concluded that the TNFα in the intestine ischemia-reperfusion injury may be mainly Derived from intestinal tissue, and mediate the pathological process of intestinal ischemia / reperfusion injury in young rats