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微繁殖具有的短周期遗传增益潜力，可实现所选林木基因型的高繁殖速率。目前，已从种子、实生苗、茎、花和木块茎等外植体建立无菌培养；至少３０个桉树种的各类组织材料诱导愈伤培养成功；从愈伤组织获得再生植株的也有１２种桉树；通过腋芽增殖，或在节上发生不定芽增殖都获得成功。以琼脂为固着剂含低生长素／分裂素比率的ＭＳ培养基被广泛的用于茎尖的分化；玻璃苗和苗的老化问题依然存在；在培养基中加入赤霉酸能刺激茎的伸长，各类培养基被用于离体物根的启动发生；悬浮培养和原生质体培养都已获成功，并从原生质获得再生植株；离体培养技术最近还被用于桉树的基因转移工程。
Micropropagation has the potential of short-cycle genetic gain to achieve high reproductive rates for selected tree genotypes. At present, aseptic culture has been established from explants such as seeds, seedlings, stems, flowers and tuber; callus culture was successfully induced by at least 30 tissue species of eucalyptus species; regenerated plants obtained from callus also had 12 Eucalyptus species; success through axillary bud proliferation or adventitious bud multiplication at nodes. MS medium with low auxin / mitogen ratio using agar as a fixing agent is widely used for differentiation of shoot tips; the aging problem of glass seedlings and seedlings still exists; adding GA3 in the medium can stimulate the elongation of the stem Long and various types of culture media are used to initiate root ex vivo development. Both suspension culture and protoplast culture have been successful and regenerated plants are obtained from the protoplasts. In vitro culture techniques have also recently been used for gene transfer engineering of eucalyptus.