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通过酰胺化反应在多壁碳纳米管(MWNTs)表面接枝双键,以L-组氨酸(L-His)为模板,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,利用表面印迹技术,在MWNTs表面制备印迹聚合物(MWNTs-MIPs).采用红外光谱,扫描电子显微镜和热重分析表征印迹聚合物的性质,结果表明MWNTs表面成功接枝了一层稳定的、厚度为35～40nm具有识别能力的印迹聚合材料.结合高效液相色谱技术,通过填充色谱柱在线色谱分析,探讨不同pH值的流动相下该印迹材料对L-His的分离行为,结果表明MWNTs-MIPs色谱柱在流动相pH=7.0时分离效果最好,能够选择性地识别L-His和D-His,分离度R为1.78,选择因子α为1.28.
The multi-walled carbon nanotubes (MWNTs) were grafted with double bond via amidation reaction. L-histidine (L-His) was used as a template, methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethyl (MWNTs-MIPs) were prepared on the surface of MWNTs by using the surface-imprinting technique by using acrylic acid ester (EGDMA) as cross-linking agent and azobisisobutyronitrile (AIBN) as initiator.Using infrared spectroscopy, scanning electron microscopy The properties of the imprinted polymer were characterized by means of reanalysis. The results showed that the surface of MWNTs was successfully grafted with a layer of imprinted polymeric material with a thickness of 35 ~ 40nm. Combined with high performance liquid chromatography (HPLC), through online chromatographic analysis, The separation of L-His from the imprinted material under different pH mobile phases was investigated. The results showed that MWNTs-MIPs had the best separation efficiency at pH = 7.0, and could selectively recognize L-His and D-His , Resolution R = 1.78, selection factor α = 1.28.