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目的 研究硫酸酯化及羧甲基化虎奶多糖的抗病毒作用效果及作用机理 ,探讨虎奶多糖应用于因柯萨奇病毒 (CVB3)导致克山病疾病预防的可能性。方法 采用病变抑制法 ,在培养的 Hela细胞上测定硫酸酯化和羧甲基化虎奶多糖对 HSV- 2和 CVB32种病毒的抑制作用 ;用 EL ISA法测定这些多糖对 HBs Ag的破坏作用。结果 2种不同取代度的羧甲基化虎奶多糖及 3种不同取代度的硫酸酯化虎奶多糖在浓度分别为 0 .5、0 .2 5、0 .12 5mg/ml时均能明显抑制有囊膜病毒 HSV - 2引起的细胞病变 ,取代度对其抗病毒活性无明显影响 ;但对无囊膜病毒 CVB3无明显的抑制作用 ,对 HBs Ag无破坏作用。结论 虎奶多糖衍生物具有很好的抗 HSV- 2病毒活性 ,其作用机理可能在于破坏病毒体的囊膜。
Objective To study the anti-virus effect and mechanism of sulfated and carboxymethylated tiger milk polysaccharide and to explore the possibility of using tiger milk polysaccharide in prevention of Keshan disease due to Coxsackievirus (CVB3). Methods Inhibition of HSV-2 and CVB-32 viruses by sulfated and carboxymethylated tiger milk polysaccharides on Hela cells was determined by the method of lesion inhibition. The destructive effects of these polysaccharides on HBsAg were determined by ELISA. Results Two kinds of carboxymethyllated tiger milk polysaccharides with different degrees of substitution and three kinds of sulfated tiger milk polysaccharides with different degrees of substitution could all be observed when the concentration was 0.5, 0.52, 0.5 and 12.5 mg / ml, respectively Suppressing the cytopathic effect caused by HSV - 2, the degree of substitution has no obvious effect on its antiviral activity. However, it has no obvious inhibitory effect on the non - enveloped virus CVB3 and has no destructive effect on HBsAg. Conclusion Tiger milk polysaccharide derivatives have good anti-HSV-2 virus activity, and its mechanism of action may be to destroy the virion envelope.