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目的:探索鼻咽癌细胞DNA合成的生物节律为临床制订鼻咽癌时辰化疗方案提供必要的参数。方法:BALB/C裸小鼠15只,置于程控的独立光照系统中(12h光照,12h黑暗)中同步化至少3周。然后双侧腋下接种人鼻咽低分化鳞癌细胞裸小鼠移植瘤CNE2,成瘤后按灯亮后3、9、15、21h(即3、9、15、21HALO)的时间点取瘤块,制成单个细胞悬液固定,染色后流式细胞仪测DNA含量。用SPSS软件系统ANOVA法检验各期细胞在4个时间点差异的显著性,用Cosinor法考察G1、S、G2/M期细胞在24h的分布是否符合余弦函数。结果:G1、S、G2/M期细胞在3、9、15、21HALO的分布随时间变化有显著性差异,G1、G2/M期细胞变化符合余弦节律。结论:移植于裸鼠的人鼻咽癌细胞的DNA合成随昼夜交替呈节律性变化。
Objective: To explore the biological rhythm of DNA synthesis in nasopharyngeal carcinoma cells to provide the necessary parameters for the preparation of the time-course chemotherapy regimen of nasopharyngeal carcinoma. METHODS: Fifteen BALB / C nude mice were synchronized for at least 3 weeks in a programmed, independent light system (12 h light, 12 h darkness). Then bilateral underarm inoculation of nasopharyngeal poorly differentiated squamous cell carcinoma cells in nude mice transplanted tumor CNE 2, after the tumor was light 3,9,15,21 h (ie 3,9,15,21 HALO) time point to take Tumors, made of a single cell suspension fixed, stained flow cytometry DNA content. SPSS software ANOVA method was used to test the significance of the difference of cells in each phase at four time points. Cosinor method was used to investigate whether the distribution of cells in G1, S, G2 / M phase met the cosine function. Results: The distribution of G1, S, G2 / M phase cells in 3, 9, 15, 21HALO had significant difference with time, and the cell changes in G1, G2 / M phase accord with cosine rhythm. Conclusion: The DNA synthesis of human nasopharyngeal carcinoma cells transplanted in nude mice changes regularly with the circadian rhythms.