论文部分内容阅读
组胺释放因子(HRF)是一组细胞因子,由单核细胞等多种细胞在抗原或丝裂原刺激下产生。HRF 可导致嗜硷细胞脱颗粒并释放组胺。巳经证明HRF 与支气管的超反应性密切相关,而这种气道的超反应性正是哮喘的特征。因此,作者设计本实验以弄清呼吸道病毒能否在体外诱导人单核细胞产生HRF。实验方法:1.取健康、非过敏体质的成人外周血,经ficoll-hypague 沉淀技术获得单核细胞,其中含有1%～3%的嗜硷细胞,并作细胞计数,将5×10~6/ml 细胞分别加入预先制备的含有流感病毒A 和含有呼吸道合胞病毒的悬液以及对照介质中,置37℃下孵化2小时,然后将细胞洗涤3次,洗涤后的细胞重悬于增添10%供者自身血浆的培养
Histamine release factor (HRF) is a group of cytokines produced by a variety of cells such as monocytes stimulated by antigen or mitogen. HRF can cause alkalophil degranulation and release histamine. It has been demonstrated that HRF is strongly associated with bronchial hyper-reactivity, which is characteristic of asthma. Therefore, the authors designed this experiment to find out whether respiratory viruses can induce human monocytes to produce HRF in vitro. Experimental methods: 1. Take healthy, non-allergic adult peripheral blood mononuclear cells obtained by ficoll-hypague precipitation technology, which contains 1% to 3% of basophils, and cell counts, the 5 × 10 ~ 6 / ml cells were added to the previously prepared suspension containing influenza virus A and respiratory syncytial virus and control medium, incubated at 37 ° C for 2 hours, then the cells were washed 3 times, the washed cells were resuspended in 10 % Donor’s own plasma culture