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目的探讨FGF21对STZ诱导的1型糖尿病小鼠心肌纤维化的影响。方法 32只8周龄雄性C57/BL6J小鼠随机分为对照组(n=6),FGF21siRNA对照组(n=6),1型糖尿病组(n=10),1型糖尿病+FGF21siRNA组(n=10)。以150 mg/kg体质量的剂量单次腹腔注射STZ建立1型糖尿病小鼠模型,造模后行FGF21siRNA给药。实验到达终点时,光镜下观察Masson染色心肌组织,电镜下观察纤维化程度,利用real-time PCR技术检测心肌组织Ⅰ型(ColⅠ)、Ⅲ型胶原(ColⅢ)、TGF-β及FGF21mRNA的表达含量。结果与正常组小鼠相比,STZ诱导的1型糖尿病小鼠心肌细胞胶原纤维增生,伴随小鼠心肌组织ColⅠmRNA、ColⅢmRNA、TGF-βmRNA、FGF21mRNA表达增加,经FGF21siRNA处理后FGF21mRNA减低,心肌细胞胶原纤维增生更明显,心肌组织ColⅠmRNA、ColⅢmRNA、TGF-βmRNA的表达进一步增加。结论 FGF21可改善STZ诱导的1型糖尿病小鼠心肌纤维化。
Objective To investigate the effect of FGF21 on myocardial fibrosis induced by STZ in type 1 diabetic mice. Methods 32 male C57 / BL6J mice of 8 weeks old were randomly divided into control group (n = 6), FGF21siRNA control group (n = 6), type 1 diabetes mellitus group (n = 10), type 1 diabetes mellitus + FGF21siRNA group = 10). The model of type 1 diabetic mice was established by single intraperitoneal injection of STZ at a dose of 150 mg / kg body weight. FGF21 siRNA was administered after modeling. At the end of the experiment, Masson-stained myocardium was observed under light microscope, and the degree of fibrosis was observed under electron microscope. The expression of ColⅠ, Col Ⅲ, TGF-β and FGF21 mRNA in myocardium was detected by real-time PCR content. Results Compared with the normal group, STZ-induced cardiomyocyte collagen fibrosis in type 1 diabetic mice was accompanied by increased expression of ColⅠmRNA, Col ⅢmRNA, TGF-βmRNA and FGF21mRNA, decreased FGF21mRNA, decreased cardiomyocyte collagen Fibrosis more obvious, myocardial tissue Col ⅠmRNA, Col ⅢmRNA, TGF-βmRNA expression further increased. Conclusion FGF21 can improve STZ-induced myocardial fibrosis in type 1 diabetic mice.