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目的 探讨乳腺癌中CyclinD1基因扩增和其蛋白表达的相关性及其临床意义。方法 应用半定量PCR和免疫组化技术 ,检测和分析了乳腺癌及其癌旁组织、乳腺良性组织及正常乳腺标本中CyclinD1基因的扩增和CyclinD1蛋白的表达情况。结果 62例乳腺癌中 ,CyclinD1基因扩增占 2 2 .6% ( 14 /62 ) ,蛋白过度表达占 48.4% ( 3 0 /62 ) ,二者有一定相关性 ,而其它各种乳腺组织的CyclinD1基因扩增及蛋白过度表达与之相比差异有显著性意义 (P <0 .0 5 )。Cy clinD1基因扩增及蛋白过度表达与乳腺癌组织学分级呈明显正相关 ,且CyclinD1蛋白过度表达还与雌孕激素受体状况呈正相关。结论 乳腺癌中CyclinD1基因扩增程度和其蛋白过度表达虽有一定相关性 ,但并不完全一致 ,说明还存在着其它导致CyclinD1蛋白过度表达的机理 ,CyclinD1蛋白的表达可能还受雌激素的调控。对乳腺癌中CyclinD1基因及其蛋白表达的检测有一定临床意义。
Objective To explore the correlation and clinical significance of CyclinD1 gene amplification and its protein expression in breast cancer. Methods Semiquantitative PCR and immunohistochemistry were used to detect and analyze CyclinD1 gene amplification and CyclinD1 protein expression in breast cancer and its adjacent tissues, benign breast tissue and normal breast specimens. Results In 62 cases of breast cancer, CyclinD1 gene amplification accounted for 22.6% ( 14/62), and protein overexpression accounted for 48.4% ( 30/62). There was a certain correlation between the two, while other types of breast tissue There was a significant difference in the amplification of CyclinD1 gene and protein overexpression (P < 0.05). CyclinD1 gene amplification and protein overexpression were positively correlated with histological grading of breast cancer. Overexpression of CyclinD1 protein was also positively correlated with estrogen and progesterone receptor status. Conclusion Although the degree of CyclinD1 gene amplification and its protein overexpression in breast cancer are related, they are not completely consistent, indicating that there are other mechanisms that lead to the overexpression of CyclinD1 protein. The expression of CyclinD1 protein may also be regulated by estrogen. . Detection of CyclinD1 gene and its protein expression in breast cancer has certain clinical significance.