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利用AFLP技术,分析了我国小麦条锈菌(Puccinia striformis f. sp. tritici)流行小种CY25,CY27,CY28,CY29,CY30,CY31和目前主要新致病类型Hybrid46致病类群类型3(Hy3),Hybrid46致病类群类型7(Hy7),水源11致病类群类型13(Sy13)以及紫外诱变菌系WV-4的DNA指纹特征,并与毒性分析进行了比较研究。主要结果如下:(1)供试菌系的AFLP指纹图谱具有很高的多态性,反映了菌系间存在丰富的遗传多样性;据此,用UPGMA聚类法建立了反映菌系间遗传距离的聚类分析树状图。(2)供试菌系的AFLP指纹多态性和毒性多态性不相关,基于这两种特征对菌系遗传关系的推断是不相同的。(3)AFLP所揭示的菌系间遗传变异度明显高于毒性分析,同时避免了毒性分析的一些缺陷,说明AFLP适合于小麦条锈菌的遗传多样性分析。(4)用DOLLOP建树法对供试菌系的进化关系作出了初步推断,结果显示供试新致病类型和原有小种可能是平行进化的。
By using AFLP technique, the epidemic races CY25, CY27, CY28, CY29, CY30, CY31 and Hygromycin (Hy) Hybrid46, a major new pathogen of Puccinia striformis f. Sp. Hybrid46, Hy7, Sy13 and UV mutagenic strain WV-4, and compared with the toxicity analysis. The main results are as follows: (1) The AFLP fingerprints of the tested strains have high polymorphism, which reflects the rich genetic diversity among the strains. Based on this, UPGMA clustering method was established to reflect the genetic Cluster analysis of distance tree. (2) AFLP fingerprinting polymorphisms and virulence polymorphisms of the tested strains were not related, and the inference of the genetic relationship between the two strains was not the same based on these two characteristics. (3) The genetic variability of strains revealed by AFLP was significantly higher than that of toxicity analysis, and some defects of toxicity analysis were avoided. It indicated that AFLP was suitable for the genetic diversity analysis of wheat stripe rust. (4) Using DOLLOP tree-making method to make a preliminary inference on the evolutionary relationship of the tested bacterial strains, the results showed that the new pathogenicity test and the original races could be parallelly evolved.