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目的:研究土壤干旱胁迫对香紫苏生理特性和光合特性的影响。方法:以香紫苏(Salvia sclareaL)的功能叶为研究对象,分实验组和对照组进行盆栽实验,在随后的5天中,分别测定两组香紫苏叶片中脯氨酸、丙二醛和叶绿素的含量,同时测定并分析蒸腾速率、气孔导度、净光合速率、细胞间隙CO2浓度的变化情况。结果:随着干旱胁迫程度的加重,香紫苏叶片内脯氨酸的含量总体趋势增加,与干旱胁迫的时间正相关;而叶片中丙二醛的含量变化不明显;干旱胁迫时,叶片内叶绿素的含量升高,在第四天时达到最大值,随干旱胁迫的加重,叶绿素含量又呈下降的趋势。其蒸腾速率变化曲线呈双峰型,随干旱时间的延长,蒸腾速率明显下降;香紫苏气孔导度变化曲线为单峰型,当干旱胁迫加重时,香紫苏气孔导度下降明显,气孔导度与胞间CO2浓度呈负相关,净光合速率变化与气孔导度变化曲线较为一致。结论:香紫苏生理因子和光合作用对干旱胁迫有一定的适应能力,但重度胁迫会对其造成严重影响。
Objective: To study the effects of soil drought stress on physiological characteristics and photosynthetic characteristics of Perilla frutescens. Methods: The functional leaves of Salvia sclarea L were used as experimental objects. The pot experiments were conducted in experimental group and control group. In the next five days, the contents of proline, malondialdehyde And chlorophyll content, and the changes of transpiration rate, stomatal conductance, net photosynthetic rate and intercellular CO2 concentration were measured and analyzed. Results: With the aggravation of drought stress, the content of proline in the leaves increased generally and correlated positively with the time of drought stress, while the content of malondialdehyde in the leaves did not change obviously. Under drought stress, Chlorophyll content increased at the fourth day reached its maximum, with the aggravation of drought stress, chlorophyll content also showed a downward trend. The transpiration rate curve showed a bimodal pattern. With the prolongation of drought time, the transpiration rate decreased significantly. The stomatal conductance curve of the scallop was single peaked. When the drought stress aggravated, the stomatal conductance decreased obviously, There was a negative correlation between conductance and intercellular CO2 concentration. The change of net photosynthetic rate and stomatal conductance were in good agreement. CONCLUSION: Physiological factors and photosynthesis of Perilla frutescens have some adaptability to drought stress, but severe stress will seriously affect it.