The Clinic Study on Synchronous Detection of HCV RNA in the Plasma and PBMC of Hepatitis C Patients

• 作 者: YE Jin LIU Wei GUO Jinsong ZENG Linglan
• 机 构: Department of Infectious Disease, Xiehe Hospital, Tongji Medical College of Huazhong University of S
• 出 处: 华中科技大学学报(医学英德文版)
• 发表日期: 2001年2期
• 关键词: chronic hepatitis C mononuclear cells peripheral blood

In order to increase the positive detection rate of HCV RNA in the patients with chronic hepatitis C (CHC), RT-PCR was used to synchronously detect HCV RNA in the plasma and peripheral blood mononuclear cells (PBMC) of 583 CHC patients with a continuously elevated level of ALT for more than one year. The results showed that the positive detection rate of HCV RNA in the plasma of the CHC patients was 19.2 %, while 24.5 % in PBMC. It was demonstrated that the positive detection rate for HCV RNA in PBMC was obviously higher than that detected in plasma. To synchronously detect HCV RNA in PBMC by using RT-PCR can increase the positive detection rate of HCV RNA in the CHC patients.