论文部分内容阅读
目的探讨锁阳对四氯化碳(CCl4)诱导的大鼠肝纤维化血细胞分型影响。方法将60只SD大鼠随机分为正常对照组、模型组、秋水仙碱组(0.2mg/kg)和锁阳组(200mg/kg),每组各15只。前6周,正常对照组大鼠用花生油,其余组大鼠2次/周,以3mL/kg的剂量,用CCl4与花生油(4∶6)混合液,皮下注射复制肝纤维化模型。6周后,秋水仙碱组与锁阳组大鼠分别以相应剂量,1次/d,灌胃,正常对照组和模型组大鼠给予等容生理盐水,持续6周。12周末,大鼠麻醉后处死,取血清,采用血细胞分析仪进行外周血常规分析。结果与模型组相比,锁阳组大鼠白细胞数目(WBC)、中性粒细胞比值(NEUT%)、嗜酸性粒细胞百分比(EOS%)、血小板分布宽度(PDW%)和红细胞分布宽度(RDW%)较模型组明显降低,淋巴细胞百分比(LYM%)、红细胞数目(RBC)、红细胞压积(HCT%)、血红蛋白含量(MCH)和平均红细胞体积(MCV)较模型组明显升高,差异有统计学意义(P<0.05)。结论锁阳对肝纤维化大鼠的血细胞分型产生了影响。
Objective To investigate the effect of Cynomorium on CCl4-induced hepatic fibrosis in rats. Methods Sixty Sprague-Dawley rats were randomly divided into normal control group, model group, colchicine group (0.2mg / kg) and Cynomorium group (200mg / kg), 15 rats in each group. In the first 6 weeks, the rats in normal control group were treated with peanut oil and the other rats were subcutaneously injected with CCl4 and peanut oil (4: 6) at a dose of 3 mL / kg twice a week for duplicating the liver fibrosis model. Six weeks later, rats in colchicine group and Cynomorium group were given isocrat saline at the corresponding dose, once a day, gavage, normal control group and model group for 6 weeks respectively. At the end of the 12th week, the rats were killed after anesthesia, the serum was collected, and the peripheral blood routine analysis was performed by using the hematology analyzer. Results Compared with model group, WBC, NEUT%, EOS%, PDW% and distribution width of erythrocytes RDW%) was significantly lower than that of the model group. The percentage of lymphocytes (LYM%), RBC, HCT%, hemoglobin (MCH) and mean corpuscular volume (MCV) The difference was statistically significant (P <0.05). Conclusion Cynomorium has an effect on the type of blood cells in rats with liver fibrosis.