亚急性重型肝炎患者TNF-α和IL-1表达及IL-4对其的调控

• 作 者: 张立煌 孙永良 石裕明 方海林 李敏伟 姚航平 刘克洲
• 机 构: 浙江大学附属第一医院传染病研究所
• 出 处: 中国免疫学杂志
• 发表日期: 1999年08期
• 关键词: 亚急性重型肝炎 TNF IL-1 肿瘤坏死因子α 白细胞介素 依赖方式 抑制作用 潜在价值 细胞因子 作用效应

目的：分析ＩＬ４对亚急性重型肝炎（亚重肝）患者外周血单个核细胞（ＰＢＭＣｓ）ＴＮＦα和ＩＬ１的调控作用，评价ＩＬ４在亚重肝治疗中的潜在价值。方法：应用细胞生物学、免疫组化和ＲＴＰＣＲ等方法测定ＰＢＭＣｓＴＮＦα、ＩＬ１的表达。结果：亚重肝患者ＰＢＭＣｓＴＮＦα、ＩＬ１表达水平均较正常人显著增高，虽然ＩＬ４ｍＲＮＡ及蛋白质的表达与正常人比较无显著性差异，但ＩＬ４ｍＲＮＡ／ＴＮＦαｍＲＮＡ和ＩＬ４ｍＲＮＡ／ＩＬ１αｍＲＮＡ的比值明显降低，ＩＬ４以剂量依赖方式明显抑制ＬＰＳ诱导的亚重肝患者ＰＢＭＣｓＴＮＦα、ＩＬ１的表达；与正常人比较，ＩＬ４对亚重肝患者ＰＢＭＣｓ的作用效应降低。结论：ＩＬ４对亚重肝ＰＢＭＣｓＴＮＦα、ＩＬ１的表达均具有明显抑制作用，在亚重肝的治疗上具有潜在的应用价值

Objective: To analyze the regulatory effect of IL4 on TNFα and IL1 in peripheral blood mononuclear cells (PBMCs) in patients with sub-acute severe hepatitis (sub-heavy liver) and to evaluate the potential value of IL4 in the treatment of subhepatic liver. Methods: The use of cell biology, immunohistochemistry and RT PCR and other methods to determine PBMCs TNF-α, IL-1 expression. Results: The expression levels of TNF-α and IL-1 in PBMCs in patients with sub-severe liver were significantly higher than those in normal subjects. Although IL-4 mRNA and protein expression were not significantly different from those in normal subjects, IL4 mRNA / TNFα mRNA and IL 4mRNA / IL  1α mRNA was significantly lower, IL  4 in a dose-dependent manner significantly inhibited LPS-induced expression of PB  MCsTNF  α, IL  1; compared with normal people, IL  4 pairs of sub-liver The effect of patients with PBMCs is reduced. Conclusion: IL  4 of subhepatic PBMCs TNF  α, IL  1 expression were significantly inhibited in the treatment of sub-heavy liver has potential value