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目的:探讨模拟高强度军事训练对大鼠肾组织Na+,K+-ATP酶活性的影响。方法:①一次性力竭运动:大鼠随机分对照组(A组)、一次性力竭运动组(B组)。B组模拟军事训练行一次性力竭跑台运动,检测运动结束即刻(B1组)及24h后(B2组)尿液指标及肾组织Na+,K+-ATP酶活性。②递增负荷跑台运动:大鼠随机分对照组(C组)、递增负荷跑台运动组(D组)。D组模拟军事训练行递增负荷跑台运动,检测4周末(C1组、D1组)和8周末(C2组、D2组)尿液指标及肾组织Na+,K+-ATP酶活性。结果:①一次性力竭运动结束即刻,B1组尿蛋白、尿N-乙酰-β-D-葡萄糖苷酶(NAG酶)较A组显著升高(P<0.05),肾组织Na+,K+-ATP酶活性显著降低(P<0.05),24h后有所恢复但仍低于A组(P<0.05)。②递增负荷跑台运动4周末D1组尿蛋白、尿NAG酶、肾组织Na+,K+-ATP酶活性均较C1组显著升高(P<0.05);8周末D2组尿蛋白、尿NAG酶较C2组显著升高,肾组织Na+,K+-ATP酶活性显著降低(P<0.05),且与尿NAG酶排泄呈负相关。结论:模拟高强度军事训练可引起大鼠肾组织Na+,K+-ATP酶活性的不同改变。
Objective: To investigate the effect of simulated high-intensity military training on Na +, K + -ATPase activity in rat kidney. Methods: (1) One-time exhaustive exercise: Rats were randomly divided into control group (A group) and once-exhausted exercise group (B group). Group B simulated a one-time force-exhausted treadmill exercise in the military training team. Urine indexes and renal tissue Na +, K + -ATPase activities were measured immediately after exercise (group B1) and after 24 hours (group B2). ② Increased load treadmill exercise: rats were randomly divided into control group (C group), increasing load treadmill exercise group (D group). Group D simulated the military training program to increase the treadmill load. The urinary indexes and the activities of Na +, K + -ATPase in renal tissue were detected at the end of 4 weeks (group C1, group D1) and 8 weeks (group C2, group D2). Results: Immediately after one - time exhaustive exercise, urinary protein and urinary NAGase in group B1 were significantly higher than those in group A (P <0.05) ATPase activity was significantly lower (P <0.05), after 24h recovery but still lower than the A group (P <0.05). (2) Compared with C1 group, urinary protein, urinary NAG enzyme and Na +, K + -ATPase activity in D1 group increased significantly (P <0.05) at the end of 4 weeks. Urinary protein and urinary NAG enzyme in D2 group C2 group was significantly increased, renal tissue Na +, K + -ATPase activity was significantly lower (P <0.05), and urinary NAG excretion was negatively correlated. Conclusion: Simulated high-intensity military training can cause different changes of Na +, K + -ATPase activity in rat kidney.