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目的:建立HPLC法测定仙蟾胶囊中华蟾酥毒基及脂蟾毒配基含量。方法:采用高效液相色谱法,色谱柱为Agilent ZORBAXSB-C18(250mm×4.6mm,5μm),流动相为乙腈(A)-水(B),梯度洗脱,流速1.0mL.min-1,检测波长296nm,柱温40℃。结果:华蟾酥毒基及脂蟾毒配基分别在4.0～100.0μg.mL-1(r=0.9999)及2.0～100.2μg.mL-1(r=0.9999)范围内线性关系良好,平均回收率(n=6)分别为101.6%(RSD=1.5%)和97.65%(RSD=1.6%)。结论:本法操作简便、准确,重复性好,可用于仙蟾胶囊的质量控制。
Objective: To establish an HPLC method for the determination of cinobufagin and resibufogenin in cefonidis capsules. Methods: The HPLC column was Agilent ZORBAXSB-C18 (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile-water (B) gradient elution at a flow rate of 1.0 mL · min- Detection wavelength 296nm, column temperature 40 ℃. Results: The concentrations of cinobufagin and resibufogenin were good linearity in the range of 4.0-100.0μg.mL-1 (r = 0.9999) and 2.0-100.2μg.mL-1 (r = 0.9999) respectively. The average recovery (n = 6) were 101.6% (RSD = 1.5%) and 97.65% (RSD = 1.6%), respectively. Conclusion: This method is simple, accurate, reproducible and can be used for the quality control of Sinodon capsule.