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目的：对１１种分枝杆菌抗原制备物用于肺结核和肺癌血清学鉴别诊断的效果进行评估。方法：采用ＥＬＩＳＡ技术分别测定１０４例肺结核患者，１０４例肺癌患者和５２例正常人血清中抗１１种分枝杆菌抗原制备物的抗体水平。结果：淋巴分枝杆菌菌体超声抗原Ｂ６ｂ检测肺结核血清抗体阳性率为８２％，显著高于抗ＰＰＤ抗体的阳性率６２％（Ｐ＜０．０５）。Ｂ６ｂ抗原检测肺癌患者血清抗体的阳性率为１．９％，远低于抗ＰＰＤ抗体的阳性检出率１５．４％（Ｐ＜０．０１）。结论：分枝杆菌抗原制备物的菌种来源、细胞培养方法和抗原制备方法对其用于血清学鉴别诊断的灵敏度和特异性，都有重要影响。其中，Ｂ６ｂ抗原用于肺结核和肺癌的鉴别诊断效果最好
OBJECTIVE: To evaluate the efficacy of 11 mycobacterial antigen preparations in the differential diagnosis of pulmonary tuberculosis and lung cancer. Methods: The serum levels of anti-11 mycobacterial antigens in 104 cases of pulmonary tuberculosis, 104 cases of lung cancer and 52 cases of normal people were determined by ELISA. Results: The positive rate of B6b antibody detection of Mycobacterium tuberculosis was 72%, which was significantly higher than that of anti-PPD antibody (62%, P <0.05). The positive rate of serum antibody of B6b antigen detection in patients with lung cancer was 1.9%, far lower than the positive detection rate of anti-PPD antibody 15.4% (P <0.01). CONCLUSION: The origin of mycobacteria and the methods of cell culture and antigen preparation of mycobacterial antigen preparations all play an important role in their sensitivity and specificity for serological differential diagnosis. Among them, B6b antigen for the differential diagnosis of tuberculosis and lung cancer best