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目的:建立多叶越南槐的HLPC指纹图谱分析方法,为科学评价和有效控制多叶越南槐药材的质量提供参考依据;并通过指纹图谱比较多叶越南槐与山豆根的相似性,为多叶越南槐与山豆根同源性研究提供参考数据。方法:采用反相高效液相色谱法,Agilent Eclipse-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.05 mol·L-1磷酸二氢钾溶液(用三乙胺调p H 6.4)梯度洗脱,流速1.0 m L·min-1,检测波长215 nm,柱温30℃,采用《中药色谱指纹图谱相似度评价系统》2004A版软件进行数据处理。结果:建立的多叶越南槐指纹图谱有14个共有峰,指认了7个共有峰;各批药材指纹图谱比较相似度大部分在0.90以上,基本符合指纹图谱研究技术的要求;多叶越南槐与山豆根指纹图谱相比较,相似度均在0.90以上。结论:该方法精密度、稳定性及重复性较好,特征性及专属性强,可以有效地用于多叶越南槐药材的质量控制,为开发和利用多叶越南槐药材资源提供科学依据,通过多叶越南槐与山豆根指纹图谱的比较,可推测出多叶越南槐与山豆根成分相似,亲缘关系相近。
OBJECTIVE: To establish HLPC fingerprinting method for the identification of Polygonum multiflori and to provide a reference for the scientific evaluation and effective control of the quality of the polygonum multiflorum S. japonica. The similarity between the polygonum multiflora Sieb. Leaf Vietnam, Sophora and root bean homologous research provide reference data. Methods: The mobile phase was eluted with acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution (reversed-phase high performance liquid chromatography with an Agilent Eclipse-C18 column (4.6 mm × 250 mm, 5 μm) 6.4) gradient elution at a flow rate of 1.0 m L · min-1 with a detection wavelength at 215 nm and a column temperature of 30 ° C. The data were processed with the 2004A version of “Chinese Medicines Chromatographic Fingerprint Similarity Evaluation System”. Results: The established multi-leaf Vietnamese Huai fingerprinting has 14 common peaks, seven common peaks were identified; the fingerprint similarity of each batch of medicinal herbs was mostly above 0.90, which basically met the requirements of fingerprinting technology; Compared with the fingerprinting of C. rubescens, the similarity was above 0.90. CONCLUSION: The method has good precision, stability and repeatability, strong specificity and specificity, and can be used effectively for the quality control of the multi-leafed Huai medicinal materials in Vietnam. It provides a scientific basis for the development and utilization of multi-leafed Huai medicinal materials in Vietnam, By comparing the fingerprints of the leaves of Sophora japonica and Sophora moorcroftiana, it could be inferred that the components of Sophora japonica and Sophora moorcroftiana were similar and the genetic relationships were similar.