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目的探讨通心络对白细胞介素1β介导的小型猪冠状动脉早期炎症反应及内膜增殖的抑制作用及可能机制。方法24头小型猪随机均分为假手术组、模型组和通心络组。假手术组和模型组喂养普通饲料,通心络组喂养普通饲料+通心络[1 g/(kg.d)]。2周后麻醉行左侧开胸手术,选择左前降支及回旋支近端管腔外径近似相同的两处节段进行仔细分离,假手术组在血管外膜包裹吸附生理盐水的纸巾,模型组和通心络组包裹吸附白细胞介素1β(2.5μg)的纸巾,术后继续以上喂养方法。2周后采用冠状动脉造影观察各组管腔狭窄情况。造影结束后处死动物,截取包裹血管段,进行病理学检查,并采用逆转录聚合酶链反应,测定各组手术处理血管段单核细胞趋化蛋白1、P选择素、E选择素、细胞间粘附分子1和血管细胞粘附分子1 mRNA的表达。结果冠状动脉造影显示,模型组冠状动脉管腔20%~30%狭窄,病理学检查可见模型组内膜增殖、炎性细胞浸润,平滑肌细胞内膜下迁移。通心络组管腔狭窄程度、内膜增殖及炎性细胞浸润现象较模型组明显减轻。逆转录聚合酶链反应示通心络组单核细胞趋化蛋白1、P选择素、E选择素、细胞间粘附分子1和血管细胞粘附分子1 mRNA表达强度低于模型组(P<0.05)。结论通心络对白细胞介素1β诱导的冠状动脉炎症反应及内膜增殖具有明显的抑制作用。通过抑制细胞因子和粘附因子的表达可能是其主要作用途径。
Objective To investigate the effect and mechanism of Tongxinluo on interleukin-1β-mediated early inflammatory response and intimal hyperplasia of miniature pigs. Methods Twenty-four minipigs were randomly divided into sham operation group, model group and Tongxinluo group. The sham group and the model group were fed normal feed, and the Tongxinluo group was fed normal feed + Tongxinluo [1 g/(kg.d)]. Two weeks later, anesthesia was performed to perform left thoracotomy. The left anterior descending branch and the proximal luminal branch with the same diameter at the proximal end of the lumen were carefully separated. The sham-operated group was wrapped with a saline-adsorbing tissue in the adventitia of the blood vessel. The group and the Tongxinluo group were wrapped in tissue paper that adsorbed interleukin 1β (2.5 μg) and continued to be fed afterwards. Two weeks later, coronary angiography was used to observe the stenosis of each group. After the end of the angiography, the animals were sacrificed, and the vascular segments were harvested for pathological examination. Reverse transcriptase polymerase chain reaction was used to determine the monocyte chemoattractant protein 1, P-selectin, E-selectin, and between cells in the vascular segment of each surgical group. Adhesion molecule 1 and vascular cell adhesion molecule 1 mRNA expression. RESULTS: Coronary angiography showed that the lumen of the coronary arteries in the model group was 20% to 30% stenosis. Pathological examination revealed intimal hyperplasia, inflammatory cell infiltration, and intimal migration of smooth muscle cells in the model group. The stenosis, intimal hyperplasia, and inflammatory cell infiltration in the Tongxinluo group were significantly less than those in the model group. Reverse transcription-polymerase chain reaction analysis showed that the mRNA expression levels of monocyte chemoattractant protein 1, P-selectin, E-selectin, intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 were lower in the Tongxinluo group than in the model group (P< 0.05). Conclusion Tongxinluo has significant inhibitory effect on interleukin-1β-induced coronary artery inflammation and intimal hyperplasia. By inhibiting the expression of cytokines and adhesion factors may be the main way of action.