,Cloning and characterization of PhPI9 involved in floral development from Phalaenopsis Orchid

• 作 者: GUO Bin DAI Wei CHEN Donghong WEI Xing MING Feng
• 机 构: Institute of Genetics,State Key Laboratory of Genetic Engineering,Research Center of Gone Diversity
• 出 处: 中国高等学校学术文摘·生物学
• 发表日期: 2007年4期
• 关键词: Phalaenopsis MADS-box gene floral development RT-PCR

In the attempt to discover new genes involved in the floral development in monoeotyledonousin species,we have cloned and characterized the homologous PISTALLATA-like (PI-like) gone from Phalaenopsis hybrid cultivar named PhPI9 (Phalaenopsis PI STILLATA # 9).The eDNA of PhPI9 has a fragment of 834 bp and has 60% identity with the PISTILATA from Arabidopsis.The deduced amino acid sequence of PhPI9 had the typical PI-motif.It also formed a subelade with other monoeot PI-type genes in phylogenetie analysis.Southe analysis showed that PhPI9 was present in the Phalaenopsis orchid genome as a single copy.Furthermore,it was expressed only in the lip of the Phalaenopsis flower and no expression was detected in vegetative organs.Thus,as a B-function MADS-box gone,PhP19 specifies floral organ identity in orchids.