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目的:研究癸甲溴铵对诱导小鼠骨髓细胞微核的形成的影响,从而为日常实验室使用癸甲溴铵提供安全评价依据。方法:对不同性别小鼠,经口给予癸甲溴铵150 mg/kg、60 mg/kg、15 mg/kg 3个剂量后,在相同时间内采集小鼠骨髓微核涂片,分析其骨髓细胞的细胞微核率以及嗜多染红细胞(PCE)和正染红细胞(NCE)的比值。结果:癸甲溴铵各剂量组小鼠PCE/NCE比值随着癸甲溴铵剂量上升而下降,但与阴性对照组比较其差异无统计学意义(P>0.05),且其比值均大于0.1;癸甲溴铵各剂量组各细胞微核率与阴性对照组比较其差异无统计学意义(P>0.05)。结论:在本实验条件下癸甲溴铵对不同性别小鼠的骨髓细胞微核未见阳性发生。
OBJECTIVE: To study the effect of decalcium bromide on the formation of micronuclei in mouse bone marrow cells, so as to provide a safety assessment basis for the daily laboratory use of decalcium bromide. Methods: Mice of different sexes were given oral doses of 150 mg / kg, 60 mg / kg and 15 mg / kg of decalcium bromide respectively, and then the bone marrow of mice were collected at the same time Cell micronucleus rate and the ratio of polychromatic erythrocyte (PCE) and positive cell (NCE). Results: Compared with the negative control group, the PCE / NCE ratio of mice in each dose of decamic bromide decreased with the dose of decahydronium bromide increasing, but the difference was not significant There was no significant difference in micronucleus rate of each cell between decamethium bromide group and negative control group (P> 0.05). Conclusion: Under the experimental conditions, decamethylammonium did not appear positive to myeloid micronuclei in different sex mice.