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分离纯化黄绿蜜环菌菌丝体粗多糖，提高多糖含量，以进一步应用于工业化生产。采用DEAE-52纤维素层析柱分离纯化黄绿蜜环菌菌丝体多糖，依次用水、0．1 mol／L NaCl、0．2 mol／L NaCl、0．4 mol／L NaCl、0．8 mol／L NaCl进行洗脱；苯酚-硫酸法测定纯化后多糖含量，测定波长490 nm，葡萄糖浓度与吸光度的回归方程为A＝6．3137X＋0．2156R2＝0．9909。黄绿蜜环菌菌丝体水体粗多糖经DEAE-52纤维素层析柱分离纯化后，多糖含量达到99％以上。经过中试试验验证提取及分离纯化工艺适用于工业化生产。“,”In order to enhance the content of polysaccharide,isolation and purification of polysaccharide of Armillaria luteo-rivens mycelium were conducted for the application to industrial production. The polysaccharide in Armillaria luteo-rivens mycelium was separated and purified by DEAE-52,and eluted gradiently by water,0. 1 mol/L NaCl,0. 2 mol/L NaCl,0. 4 mol/L NaCl and 0. 8 mol/L NaCl;the content of polysaccharide was measured by the phenol-sulfuric acid method,determined with wavelength of 490 nm. The linear relationship of glucose concentration was good with regression equation A=6. 3137X+0. 2156(R2 =0. 9909). The content of polysaccharide exceeded 99% after isolation and purifica-tion. The results showed that the process of extraction,separation and purification was suitable for industrial production.