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依据液相色谱中溶质计量置换保留模型及线性参数 log I(与 1mol溶质对固定相的亲和势大小有关的常数 ) ,通过作图得知非极性和极性小分子溶质及生物大分子的 log I与绝对温度的倒数 1/T,以及小分子溶质的log I与其在正辛醇 -水中分配系数的对数 log Po/ w呈线性关系 ,从两方面进一步证明了 log I具有热力学平衡常数的性质。基于小分子溶质、生物大分子的 log I和分配系数大小的差别 ,对两者在反相液相色谱中的保留对柱长的依赖关系给予了定量的说明
According to the retention model of solute mass displacement in liquid chromatography and the linear parameter log I (a constant related to the affinity of 1 mol solute to the stationary phase), it is known that the nonpolar and polar small molecule solutes and biological macromolecules Log I and absolute reciprocal temperature 1 / T, and the log I of the small molecule solute and its logarithm log Po / w of the partition coefficient in n-octanol-water, further prove that log I has a thermodynamic equilibrium The nature of the constant. Based on the difference between the log I and the partition coefficients of the small molecule solutes and biological macromolecules, the quantitative relationship between the column retention and the column length dependence of the two in reversed-phase liquid chromatography