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目的:优选丹参活血化瘀有效组分的最佳提取纯化工艺。方法:以隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA,作为丹参活血化瘀作用脂溶性有效组分的指标成分,丹酚酸B为水溶性有效组分的指标成分,考察不同的提取、纯化方式,并优选出提取纯化的最佳工艺条件。结果:丹参中脂溶性的总丹参酮有效组分采用乙醇渗漉法提取,最佳工艺为6倍量的乙醇以5mL·min-1·Kg-1的速度渗漉提取;水沉法纯化,最佳条件为20倍生药材量的水进行沉淀。丹参中水溶性的总酚酸有效组分采用水提法,对丹参渗漉醇提后的药渣进行水提取,最佳工艺为8倍量水提取2次每次1小时;大孔树脂纯化,最佳条件为AB-8型大孔吸附树脂,0.5g生药/mL样品以1mL·min-1的流速吸附,最大上样体积为60mL,再以10倍药材量,流速1mL·min-1的30%乙醇洗脱。结论:优选最佳工艺得到的丹参活血化瘀有效部位中总丹参酮和总酚酸含量均较高,且工艺简单,适宜工业化生产。
Objective: To optimize the extraction and purification of the active components of salvia miltiorrhiza. Methods: Cryptotanshinone, tanshinone Ⅰ and tanshinone ⅡA were used as the index components of fat-soluble active components of salvia miltiorrhiza. Blood salvianolic acid B was the index component of water-soluble active components, and different extraction and purification methods were investigated. Optimum extraction and purification of the best process conditions. Results: The total soluble components of total tanshinone in Salvia miltiorrhiza were extracted by ethanol percolation method. The optimum extraction conditions were as follows: 6 times the amount of ethanol was percolated and extracted by 5mL · min-1 · Kg-1; Good conditions for 20 times the amount of crude drug precipitation. Salvia miltiorrhiza water-soluble total phenolic acids effective component of water extraction, danshen bleeding alcohol dregs after water extraction, the best technology for 8 times the amount of water extraction twice each 1 hour; macroporous resin purification , The optimum conditions were AB-8 macroporous resin, 0.5g crude drug / mL sample was adsorbed at the flow rate of 1mL · min-1, the maximum loading volume was 60mL, and then 10 times the amount of crude drug, flow rate 1mL · min-1 Of 30% ethanol. CONCLUSION: The content of total tanshinone and total phenolic acids in the effective part of Salvia miltiorrhiza Bunge, which is obtained by the optimal process, is high, and the process is simple and suitable for industrial production.