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七叶素是一种三萜葡糖甙类混合物,天然情况下主要存在于欧洲七叶树的种子中。该成分广泛用于各种外周血管疾病的预防和治疗。为了考察由体外培养产生七叶素的可能性,采用高效液相法对欧洲七叶树叶外植体(explants)产生的愈伤组织和胚状体中七叶素进行了含量测定,同时对组织分化和次生代谢物的生物合成之间的关系作了讨论。组织培养外植体来自无菌的合子胚以0.9％Difco Bactko琼脂和3％蔗糖作体外培养所得的欧洲七叶树秧苗长成初生叶,为获得愈伤组织,外植体置于补加了激动素(K)、萘乙酸(N)、2,4-D(D)和3％蔗糖的MS培养
Escin is a triterpene glycoside mixture that is found naturally in the seeds of horse chestnut. This ingredient is widely used in the prevention and treatment of various peripheral vascular diseases. In order to investigate the possibility of producing aescin in vitro, the content of aescine in callus and embryoid body produced by explants of Aesculus hippocastanum was determined by high performance liquid chromatography (HPLC) The relationship between differentiation and biosynthesis of secondary metabolites is discussed. Tissue Culture Explants from Sterile Zygotic Embryos New Zealand white horse chestnut seedlings grown in vitro with 0.9% Difco Bactko agar and 3% sucrose grow into primary leaves. To obtain callus, the explants are placed on an additional Kinetin (K), NAA (N), 2,4-D (D) and 3% sucrose